5-FAM

2020-05-25 14:00:08      点击:

5-FAM荧光试剂(fluorescence reagent)一种有机试剂,能与荧光较弱或不显荧光的物质共价或非共价结合形成荧光的配合物。如8-羟基喹啉、黄酮醇、石榴茜素R、桑色素等。在它们的分子中大多含有芳香环或杂环。主要用于无机元素的荧光测定,例如,铍、铝、硼、镓、硒、镁以及某些稀土元素。对有机化合物也能对它们所发的荧光起加强、稳定和提高选择性的作用。


 5-FAM是一种可用作酸碱指示剂的荧光染料,5-FAM具有极强的浓度依赖性并且可以显示较强的荧光,可通过羧基和氨基的反应标记蛋白质,核甘酸和多肽。


 5-FAM is the purified single isomer of carboxyfluorescein. It is one of the most popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. It has been predominantly used to develop a variety of green fluorescent peptides that can be excited with the 488 nm line of the Ar laser. It has also been used to prepare various small fluorescent molecules.

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